"Transgenic Plants"

Methods and Protocols

edited by L. Peña

Humana, August, 2004, 286 pages. $159.00 + shipping ($5.00, U.S. or $15.00 Elsewhere)

A collection of readily reproducible techniques for the genetic transformation and regeneration of plants. The authors cover the most commonly used transformation systems for the regeneration of whole transgenic plants. Among the techniques discussed for the detection of transgenes are quantitative real-time PCR, RT-PCR, in situ hybridization, FISH, and TAIL-PCR. Risk assessment methods offer the opportunity to study Agrobacterium persistence in plant tissues and to investigate the possibility of transgene dispersal. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Contents:
1.  Transgenic Plants: An Historical Perspective, Herrera-Estrella, Luis et al.
2.  Plant Transformation: Agrobacterium-Mediated Gene Transfer, Dandekar, A et al
3.  Production of Hairy Root Cultures and Transgenic Plants by Agrobacterium rhizogenes-Mediated Transformation, Christey, Mary C.; Braun, Robert H.
4.  Stable Transformation of Plant Cells by Particle Bombardment/Biolistics, Kikkert, J. R. et al
5.  Electroporation: Introduction and Expression of Transgenes in Plant Protoplasts Fisk, Henry J.; Dandekar, Abhaya M.
6. Floral Dip: Agrobacterium-Mediated Germ Line Transformation, Clough, Steven J.
7. Production of Transgenic Crops by the Floral-Dip Method, Curtis, Ian S.
8. Chloroplast Genetic Engineering to Improve Agronomic Traits, Daniell, Henry et al
9. Organogenesis From Transformed Tomato Explants, Frary, Anne; Van Eck, Joyce
10. Genetic Transformation of Conifers Utilizing Somatic Embryogenesis Klimaszewska, Krystyna; Rutledge, Robert G.;
11. Regeneration of Transgenic Cassava From Transformed Embryogenic Tissues Zhang, Peng; Puonti-Kaerlas, Johanna
12. Genetic Transformation of Mature Citrus Plants Cervera, Magdalena; Juárez, José; Navarro, Luis; Peña, Leandro
13. Selectable Markers: Antibiotic and Herbicide Resistance, Goodwin, Julia L. et al.
14. Histochemical and Fluorometric Assays for uidA (GUS) Gene Detection, Cervera,M
15. Green Fluorescent Protein Quantification in Whole Plants, Halfhill, M.D. et al.
16. Positive Selection, Wenck, Allan; Hansen, Geneviève
17. Elimination of Marker Genes From Transgenic Plants Using MAT Vector Systems Ebinuma, Hiroyasu; Sugita, Koichi; Endo, Saori; Matsunaga, Etsuko; Yamada, Keiko
18.  Simple and Efficient Removal of Marker Genes From Plastids by Homologous Recombination, Day, Anil; Kode, Vasumathi; Madesis, Panagiotis; Iamtham, Siriluck
19.  The Study of Transgene Copy Number and Organization, Ingham, David J.
20.  Analysis of Gene Expression in Transgenic Plants, Page, Andrew F. et al.
21.  Transgene Integration: Use of Matrix Attachment Regions, Allen, George C.et al.
22.  Fluorescence In Situ Hybridization to Localize Transgenes in Plant Chromosomes Harwood, Wendy A., et al.
23. Amplification of Genomic Sequences Flanking T-DNA Insertions by Thermal Asymmetric Interlaced Polymerase Chain Reaction, Liu, Yao-Guang, et al.
24. Agrobacterium Persistence in Plant Tissues After Transformation, Cubero, J., et al.
25. Transgene Dispersal Through Pollen, Hudson, Laura C., et al.
26.  Transgenic Crops: The Current and Next Generations, Dunwell, Jim M.
27.  Comparative Development and Impact of Transgenic Papayas in Hawaii, Jamaica, and Venezuela, Fermín, Gustavo, et al.

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