"Techniques for Molecular Biology"

CONTENTS:

Definition: Structure and Expression of a Eukaryote Gene Coding for an mRNA and a Protein • Parameters of Description of a Genome • Sequencing of Entire Genomes

Vectors and Cloning: Restriction Enzymes • Electrophoresis of Nucleic Acids • Description of a Plasmid and a Phagemid • Description of a Bacteriophage and a Cosmid • Description of a YAC and Other Large Vectors • Molecular Cloning • Genetic Transformation of Bacteria and Yeasts

Labelling of Nucleic Acids and Hybridization: DNA Labelling • Molecular Hybridization • In situ Hybridization of mRNA

DNA Libraries and Screening: Construction of a Genomic DNA Library • Construction of a cDNA Library • Screening of a Library • Differential Display: Subtractive Libraries, AFLP-cDNA • Differential Display RT-PCR: mRNA Screening • Differential Display SSH: Suppression Subtractive Hybridization • Differen-tial Screening by RDA: Representational Difference Analysis • EST: Expressed Sequence Tags • DNA Microarrays: DNA Microchips, cDNA Filters

Characterization of a Gene: DNA Sequencing • PCR: Polymerase Chain Reaction • RACE: Rapid Amplification of cDNA Ends • Genome Walking by PCR • RT-PCR: Reverse Transcriptase PCR • In vitro Transcription • Determination of Transcription Initiation Site • Functional Analysis of Promoters • Gel Retarda-tion • DNAse I Footprinting

Genetic Transformation of Euaryotes: Genetic Transformation of Plants by Agrobacterium tumefaciens • Direct Gene Transfer into Plant Protoplasts • Direct Gene Transfer by Biolistics • Genetic Transformation of Animal Cells • Cloning of Animals • Transient Expression

Analysis of Gene Function: Recombinant Proteins • Baculoviruses of Insects, Vectors of Expression of Foreign Genes • Double Hybrid System • Directed Mutagenesis • Mutation Complementation in Yeast • Knock-out of Genes • Gene Tagging • RNA Interference

Polymorphism of a Genome: Molecular Markers • Genetic and Physical Maps • PFGE: Pulse Field Gel Electrophoresis • RFLP: Restriction Fragment Length Polymorphism • RAPD: Random Amplified Polymorphic DNA • AFLP: Amplified Fragment Length Polymorphism • RETROMARKERS • SSCP: Single Strand Conformation Polymorphism • DGGE: Denaturing Gel Gradient Electrophoresis • SNP: Single Nucleotide Polymorphism • Simple Sequence Repeats (SSR): Microsatellites

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