"Recombinant Proteins From Plants"

Methods and Protocols

Faye, Loïc; Gomord, Véronique (Eds.)

2009, XVIII, 358 p. 59 illus., 8 in color., Hardcover. Price: $109.00 + shipping and handling ($8.00, U.S. or $20.00 Elsewhere)

The past decade has seen the emergence of plants as a viable alternative to the current production systems for therapeutic proteins. In Recombinant Proteins from Plants, expert researchers explore plants and their potential for the production of increasingly safe, high quality and biologically active complex recombinant pharmaceutical proteins. Chapters present several production platforms, focusing on the largest and most important group of biotechnological products in clinical trials: antibodies and their derived fragments with acknowledged potential for immunotherapy in humans. Due to the shortage of step by step protocols that can be used easily by beginners, this volume aims to fill the void with several chapters of detailed instructions for using the main plant expression systems. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Recombinant Proteins from Plants serves as an ideal guide for those new and seasoned plant scientists, pharmaceutical scientists, and molecular and cellular biologists interested in molecular pharming.

CONTENTS:

1. From Neanderthal to Nanobiotech:  from plant potions to pharming with plant factories 2. Cowpea Mosaic Virus-based systems for the expression of antigens and antibodies in plants. 3. Transient expression of antibodies in plants using syringe agroinfiltration 4. Rapid system for evaluating bioproduction capacity of complex pharmaceutical proteins in plants 5. Production and localization of recombinant pharmaceuticals in transgenic seeds 6. Production of antibody fragments in Arabidopsis seeds 7. Production of plantibodies in Nicotiana plants 8. Physcomitrella patens: a non-vascular plant for recombinant protein production 9. Production of recombinant proteins in suspension-cultured plant cells 10. Chloroplast-derived vaccine antigens and biopharmaceuticals: Protocols for expression, purification or oral delivery and functional evaluation 11. Protein body induction: a new tool to produce and recover recombinant proteins in plants 12. A case study for plant-made pharmaceuticals comparing different plant expression and production systems 13. Glycosylation of antibody therapeutics: optimisation for purpose 14. N-Glycosylation of Plant Recombinant Pharmaceuticals 15. Companion protease inhibitors to protect recombinant proteins in transgenic plant extracts 16. Strategies for improving vaccine antigens expression in transgenic plants. Fusion to carrier sequences 17. Immunomodulation of plant function by in vitro selected single-chain Fv intrabodies 18. On-chip detection of low-molecular-weight recombinant proteins in plant crude extracts by SELDI-TOF MS 19. Assessing the Risk of Undesirable Immunogenicity/Allergenicity of Plant-Derived Therapeutic Proteins 20. Biosafety, Risk Assessment and Regulation of Plant-made Pharmaceuticals

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