"Microbial Contamination of Plant Tissue Cultures"

Volume 4 of Recent Advances in Plant Tissue Culture

by Edwin B. Herman, Editor, Agricell Report

Microbial Contamination of Plant Tissue Cultures is the fourth in a series, based on articles published in Agricell Report, that reports developments in a specific area of plant tissue culture.

In order to make it easy for the reader to obtain further information on material covered in the book, a directory consisting of full addresses of cited authors is included. The book also includes a list of recent symposia, a taxonomic index and an index of antimicrobial agents.

Partial Contents: 1. Novel approaches to controlling contamination: Production of antimicrobial agents by plant cell cultures, screening for elicitors that stimulate phytoalexin yield. 2. Plant tissue culture contaminants: Identification, screening, sources, vitropaths, latent contamination. 3. Sterilization of seeds. 4. Disinfestation of explants: Laser radiation, running water treatment, hot water treatment, double disinfestation, sulfite pads, bleach, pre-forcing, internal disinfestation. 5. Sterilization of medium: Autoclaving and role of activated charcoal, microwaving, diethylpyrocarbonate. 6.Elimination of microbial contaminants: Density gradient centrifugation, flexible container system, repeated subculture, low free-water medium, acidification, egg white lysozyme. 7. Use of antibiotics: Effectiveness, elimination of latent contamination, phytotoxicity, stimulation of regeneration and growth, use of antibiotic discs. 8. Viral elimination: Petal culture, chemotherapy and combined therapies, multiple lateral shoot technique. 9. Control of nematodes. 10. Non-axenic plant tissue cultures: Non-sterile micropropagation, coculture with bacteria and fungi, microbial promotion of morphogenesis and embryogenesis, microbial stimulation of secondary metabolite yields. 11. Possibilities and opportunities for non-axenic tissue culture.

Publication: April, 1996, 84 pp., softbound. Price: US$ 73.00 (Shipping and handling: $8.00 for U.S. delivery, $15.00 elsewhere). The price includes a copy of the October, 1996 issue of Agricell Report, that features highlights of the 1996 Symposium on Bacterial and Bacteria-Like Contaminants of Plant Tissue Cultures, held in Cork Ireland.

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