"Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants"

Series: Methods in Molecular Biology , Vol. 547

edited by Jain, S. Mohan; Saxena, Praveen K.

2009, XVIII, 350 p. 113 illus., Hardcover, Price: $110.00 + shipping and handling ($8.00, U.S. or $20.00 Elsewhere)

Given the vital and far-reaching applications of medicinal plant metabolites worldwide, the quality and consistency of the products as well as the very survival of various species are of the utmost importance. In Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, expert researchers provide detailed, step-by-step protocols for the establishment of in vitro cultures of key medicinal plants, their mass multiplication in a controlled environment, and step-wise secondary metabolite analysis, genetic transformation, large-scale metabolite production in a bioreactor, and molecular markers. In addition, many of these protocols will provide a basis for much needed efforts of in vitro germplasm conservation or cryopreservation of medicinal plant species at the brink of extinction as well as efforts to protect them from the adverse impact of rapid climatic changes. As a volume in the Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Contents

PART I: MICROPROPAGATION AND CHEMICAL ANALYSIS

1. Establishment of Adventitious Root Cultures of Echinacea purpurea for the Production of Caffeic Acid Derivatives 2. In Vitro Propagation of Rauwolfia serpentina Using Liquid Medium, Assessment of Genetic Fidelity of Micropropagated Plants 3. Medicinal Properties, In Vitro Protocols and Secondary Metabolite Analyses of Scots Pine 4. Saussurea medusa Cell Suspension Cultures for Flavonoid Production 5. Large-scale In Vitro Multiplication of Crataeva nurvala 6. Bilberry In Vitro Protocols and Analyses of Phenolic Compounds 7. In Vitro Propagation of Two Tuberous Medicinal Plants: Holostemma ada-kodien and Ipomoea mauritiana 8. In Vitro Production of Gymnemic Acid from Gymnema sylvestre (Retz) R. Br. Ex Roemer and Schultes Through Callus Culture Under Abiotic Stress Conditions 9. Establishment of Plant Regeneration and Cryopreservation System from Zygotic Embryo-Derived Embryogenic Cell Suspension Cultures of Ranunculus kazusensis 10. In Vitro Culture and Secondary Metabolite Isolation in Bryophytes 11. Micropropagation and In Vitro Conservation of Vanilla 12. Protocol for In Vitro Regeneration and Marker Glycoside Assessment in Swertia chirata Buch-Ham 13. Protocols for Establishment of an In Vitro Collection of Medicinal Plants in the Genus Scutellaria 14. Protocols for In Vitro Culture and Phytochemical Analysis of Phyllanthus Species (Euphorbiaceae) 15. In Vitro Clonal Propagatin of Asparagus racemosus, a High Value Medicinal Plant 16. Micropropagation of Penthorum chinense Through Axillary Buds

PART II: TRANSGENIC APPROACHES

17. Spontaneous Plant Regeneration and Production of Secondary Metabolites from Hairy Root Cultures of Centaurium erythraea Rafn 18. Transgenic Hypericum perforatum 19. Agrobacterium-Mediated Transformation of Ruta graveolens L. 20. Gene Expression Profiling in Taxus baccata L. Seedlings and Cell Cultures 21. Catapol Production in Chinese Foxglove (Rehmannia glutinosa Libos.) Hairy Roots Transformed with Agrobacterium rhizogenes ATCC15834

PART III: MOLECULAR MARKERS AND MICROSATELLITES

22. Identification of Medicinal Plants and Plant Sequences: A Multiplexed MLPA Assay 23. Isolation of Microsatellites from Catharanthus roseus (L.) G. Don Using Enriched Libraries PART

IV: BIOTRANSFORMATION, BIOREACTORS, AND METABOLOMICS

24. Production of Cinnamyl Glycosides in Compact Callus Aggregate Cultures of Rhodiola rosea Through Biotransformation of Cinnamyl Alcohol 25. Spearmint Plantlet Culture System as a Means to Study Secondary Metabolism 26. Bioreactor Production of Secondary Metabolites from Cell Cultures of Periwinkle and Sandalwood 27. Camptothecin Production by In Vitro Cultures and Plant Regeneration in Ophiorrhiza Species 28. Metabolomic Analysis of Ocotea odorifera Cell Cultures: A Model Protocol for Acquiring Metabolite Data 29. The Production of 9-methoxycanthin-6-one from Callus Cultures of (Eurycoma longifolia Jack) Tongkat Ali

PART V: ALTERED GRAVITY AND BIOTECHNOLOGY

30. Plant Secondary Metabolism in Altered Gravity 31. The Role of Biotechnology in the Production of the Anticancer Compound Podophyllotoxin

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