"Plant Secondary Metabolism Engineering"

Methods and Applications

Fett-Neto, Arthur Germano (Ed.)

1st Edition., 2010, X, 326 p. 65 illus., 6 in color.
Price: $139.00 (Shipping and handling: $8.00 for U.S. delivery, $20.00 elsewhere).

In Plant Secondary Metabolism Engineering: Methods and Applications, expert researchers provide detailed practical information on some of the most important methods employed in the engineering of plant secondary metabolism pathways and in the acquisition of essential knowledge in performing this activity, including the significant advances and emerging strategies. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Table of Contents:

1. Plant Secondary Metabolism and Challenges in Modifying Its Operation: An Overview
2. Suppression Subtractive Hybridization as a Tool to Identify Anthocyanin Metabolism-Related Genes in Apple Skin
3. Identification of Regulatory Protein Genes Involved in Alkaloid Biosynthesis Using a Transient RNAi System
4. Site-Directed Mutagenesis and Saturation Mutagenesis for the Functional Study of Transcription Factors Involved in Plant Secondary Metabolite Biosynthesis
5. Isolation of Proteins Binding to Promoter Elements of Alkaloid Metabolism-Related Genes Using Yeast One-Hybrid
6. Modulation of Carotenoid Accumulation in Transgenic Potato by Inducing Chromoplast Formation with Enhanced Sink Strength
7. Over-expression of Rate-Limiting Enzymes to Improve Alkaloid Productivity 8. Microbial Expression of Alkaloid Biosynthetic Enzymes for Characterization of Their Properties
9. Producing a Recombinant Flavin-Containing Monooxygenase from Coffea arabica in Escherichia coli for Screening of Potential Natural Substrates
10. Efficient Production of Active Form Recombinant Cassava Hydroxynitrile Lyase Using Escherichia coli in Low-Temperature Culture
11. Introduction of the Early Pathway to Taxol Biosynthesis in Yeast by Means of Biosynthetic Gene Cluster Construction Using SOE-PCR and Homologous Recombination
12. Biocatalytic Synthesis of Tritium (3H)-Labelled Taxa-4(5),11(12)-diene, the Pathway Committing Precursor of the Taxoid Diterpenoids
13. USER Cloning and USER Fusion: The Ideal Cloning Techniques for Small and Big Laboratories
14. Enrichment of Carotenoids in Flaxseed by Introducing a Bacterial Phytoene Synthase Gene
15. Metabolic Engineering by Plastid Transformation as a Strategy to Modulate Isoprenoid Yield in Plants
16. Engineering High Yields of Secondary Metabolites in Rubia Cell Cultures Through Transformation with Rol Genes
17. Flow Cytometric Methods to Investigate Culture Heterogeneities for Plant Metabolic Engineering
18. Phenylpropanoid Biosynthesis in Leaves and Glandular Trichomes of Basil (Ocimum basilicum L.)
19. Fusion with Fluorescent Proteins for Subcellular Localization of Enzymes Involved in Plant Alkaloid Biosynthesis
20. Immunohistochemical Localisation of a Putative Flavonoid Transporter in Grape Berries
21. Electrogenic Bromosulfalein Transport in Isolated Membrane Vesicles: Implementation in Both Animal and Plant Preparations for the Study of Flavonoid Transporters
Subject Index

Visa, Mastercard or American Express Card is now accepted

To Order Books

Return to Main Page

Agritech Publications/Agricell Report

P.O. Box 255
Shrub Oak, NY 10588, U.S.A.
Phone/Fax: (914) 528-3469
E-mail: Agritech@AgritechPublications.com