"Plant Functional Genomics"

Methods and Protocols

by Grotewold, Erich (The Ohio State University, Columbus, OH)

Humana Press, August 2003, 472 pages. $125.00 + shipping ($5.00, U.S. or $15.00 Elsewhere)

Erich Grotewold has assembled a team of leading plant scientists to describe in detail the most commonly used methods for investigating plant gene function in a wide variety of plants, during plant pathogen interactions, and even in algae. These readily reproducible protocols include computational, molecular, and genetic methodologies designed for both general and specific problems. Here the reader will learn about powerful computational and statistical tools to help predict gene function either on the basis of comparative genomics, or from the analysis of complex genome sequences. Numerous loss-of-function and gain-of-function techniques for discovering gene function are presented in step-by-step detail.

CONTENTS

1. An Improved Method for Plant BAC Library Construction

2. Constructing Gene-Enriched Plant Genomic Libraries Using Methylation Filtration Technology

3. RescueMu Protocols for Maize Functional Genomics

4. Precious Cells Contain Precious Information: Strategies and Pitfalls in Expression Analysis from a Few Cells

5. Combined ESTs from Plant–Microbe Interactions: Using GC Counting to Determine the Species of Origin

6. Computer Software to Find Genes in Plant Genomic DNA

7. Genomic Colinearity as a Tool for Plant Gene Isolation

8. Using Natural Allelic Diversity to Evaluate Gene Function

9. Quantitative Trait Locus Analysis as a Gene Discovery Tool

10. Transposon Tagging Using Activator (Ac) in Maize

11. T-DNA Mutagenesis in Arabidopsis

12. Physical and Chemical Mutagenesis,

13. High-Throughput TILLING for Functional Genomics

14. Gene and Enhancer Traps for Gene Discovery

15. High-Throughput TAIL-PCR as a Tool to Identify DNA Flanking Insertions

16. Custom Knock-Outs with Hairpin RNA-Mediated Gene Silencing

17. Virus-Induced Gene Silencing

18. Exploring the Potential of Plant RNase P as a Functional Genomics Tool

19. Maintaining Collections of Mutants for Plant Functional Genomics,

20. Vector Construction for Gene Overexpression as a Tool to Elucidate Gene Function

21. T-DNA Activation Tagging

22. Expression Profiling Using cDNA Microarrays

23. Open Architecture Expression Profiling of Plant Transcriptomes and Gene Discovery Using GeneCalling® Technology

24. Proteomics as a Functional Genomics Tool

25. Metabolite Profiling as a Functional Genomics Tool

26. Growth Stage-Based Phenotypic Profiling of Plants

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