"Handbook of Industrial Cell Culture"

Mammalian, Microbial and Plant Cells

edited by Vinci, Victor A. (Eli Lilly and Company, Indianapolis, IN) and Parekh, Sarad R. (Dow AgroSciences LLC, Indianapolis, IN)

Humana Press, December 2002, 546 pages. $204.00 + shipping ($5.00, U.S. or $15.00 Elsewhere)

A diverse team of researchers, technologists, and engineers describe, in simple and practical language, the major current and evolving technologies for improving the biocatalytic capabilities of mammalian, microbial, and plant cells. The authors present state-of-the-art techniques, proven methods, and strategies for industrial screening, cultivation, and scale-up of these cells, and describe their biotech and industrial uses. Special emphasis is given to the solving critical issues encountered during the discovery of new drugs, process development, and the manufacture of new and existing compounds. Other topics include recombinant protein expression, bioinformatics, high throughput screening, analytical tools in biotechnology, DNA shuffling, and genomics discovery.

Contents:

Part I. Mammalian Cell Culture:

Analysis and Manipulation of Recombinant Glycoproteins Manufactured in Mammalian Cell Culture, Nigel Jenkins; Genetic Approaches to Recombinant Protein Production in Mammalian Cells, Peter P. Mueller, Dagmar Wirth, Jacqueline Unsinger, and Hansjörg Hauser; Protein Expression Using Transgenic Animals, William H. Velander and Kevin E. van Cott; Mammalian Cell Culture: Process Development Considerations, Steven Rose, Thomas Black, and Divakar Ramakrishnan

Part II. Microbial Cell Culture

Natural Products: Discovery and Screening, Matthew D. Hilton; Genetic Engineering Solutions for Natural Products in Actinomycetes, Richard H. Baltz; Culture Medium Optimization and Scale-Up for Microbial Fermentations, Neal C. Connors

Part III. Plant Cell Culture

Functional Genomics for Plant Trait Discovery, Sam Reddy, Ignacio M. Larrinua, Max O. Ruegger, Vipula K. Shukla, and Yuejin Sun; Molecular Tools for Engineering Plant Cells, Donald J. Merlo; Plant Cell Culture: A Critical Tool for Agricultural Biotechnology, Joseph F. Petolino, Jean L. Roberts, and Ponsamuel Jayakumar; Expression of Recombinant Proteins via the Plastid Genome, Jeffrey M. Staub; Oleosin Partitioning Technology for Production of Recombinant Proteins in Oil Seeds, Maurice M. Moloney.

Part IV. Critical Tools for Biotechnology

Genome Sequencing and Genomic Techniques in Drug Discovery and Development, Lawrence M. Gelbert; Proteomics, Gerald W. Becker, Michael D. Knierman, Pavel Shiyanov, and John E. Hale; Metabolic Flux Analysis, Modeling, and Engineering Solutions, Walter M. van Gulik, Wouter A. van Winden, and Joseph J. Heijnen; Advances in Analytical Chemistry for Biotechnology: Mass Spectrometry of Peptides, Proteins, and Glycoproteins, Jeffrey S. Patrick; DNA Shuffling for Whole Cell Engineering, Steve del Cardayre and Keith Powell; Cell Culture Preservation and Storage for Industrial Bioprocesses, James R. Moldenhauer Index

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