"Genetic Modification of Plants: Methods and Applications 2005-2009"

edited by Edwin B. Herman

April, 2009, Approx 150 pp., Spiralbound. $129.00 + shipping ($8.00, U.S. or $15.00 Elsewhere)

PARTIAL CONTENTS:

CHAPTER 1. METHODS FOR BACTERIUM-MEDIATED TRANSFORMATION

I. Methods for Improving Agrobacterium-Mediated Transformation A. Buffering of Coculture Medium B. High Concentrations of Hypochlorite and Tween C. Mechanical Wounding: Vortexing, Needles, Scalpel, Macerating Enzymes, Compressed Air D. Cotyledonary Node Method E Use of Disarmed A. rhizogenes F. Plasmolysis G. Lipoic Acid H. Pretreatment with Centrifugation and Heat I. Okadaic Acid or Trifluoperazine J. Paromomycin and Cryopreserved Cells K. Advantages of Liquid Culture L. Use of Embryogenic Calli M. Minimally Sterile Method N. Effects of Light O. Thiol Compounds P. High Agar Levels Q. Desiccation R. Sonication S. Calcium-free Medium T. Ethylene Inhibitor U Antinecrotics and Antioxidants V. Naringen W. Vacuum Infiltration X. Agroinjection Y. Magnifection II. Spaghetti-Like Form of Agrobacterium: Possible Role in the Infection Process III. Elimination of Agrobacterium from Cocultures: A Novel Antibiotic, Overgrowth-Control Antibiotics IV. Transformation of Plants by Non-Agrobacterium

CHAPTER 2. METHODS FOR DIRECT TRANSFORMATION

I. Electroporation II Vertically-Aligned Nanofibers III. Silicon Carbide Whiskers IV. Particle Gun Transformation V. Methods for Improving Particle Gun Transformation A. Low-Pressure Gene Gun B. Use of Protamine C. Equipment Improvements D. Osmotic Pretreatment E. Use of Functionalized Particles F. Heat and Isopropanol Pretreatment G. Bombardment with Bacteria H. Accelerated Nanoparticles I. Magnetic Particles VI. Laser-Mediated Transformation VII. Ovary Wound Dipping and Flower Dipping VII. Bio-Bead Transformation IX. Floral Spray Transformation X. PEG-Mediated Transformation XI. Neutron Beam-Mediated Transformation XII. Microinjection XIII. ACE Gene Delivery and Expression System XIV.Importance of Cell or Tissue Type Used XV. Electrophoresis

CHAPTER 3. COMPARISON OF METHODS FOR GENETIC TRANSFORMATION

I. Use of Protamine II. Particle Bombardment vs. Agrobacterium III. Three Maize Transformation Methods IV. Three Poinsettia Transformation Methods V. Four Citrus Transformation Methods VI. Use of Dehydration/Rehydration VII. Use of Stem Thin Cell Layers VIII. Effect of Carbon Source

CHAPTER 4. REGULATION OF PLANT GENE EXPRESSION

I. New Promoters Control Gene Expression II. Matrix Attachment Regions (MARs): A. Enhancement of Transgene Expression B. Reduction in Gene Expression Variation C. Use of Synthetic MARs D. MARs Mechanism of Action III. Photoperiod Regulation of the Camv-35s Promoter IV. Plant Genes That Respond to Sound V. Embryogenesis Stage Affects Gene Expression VI. Ethylene Vapor Induces Gene Expression VII. Agrodrench Inoculation Method for Virus-induced Gene Silencing

CHAPTER 5. STRATEGIES FOR SELECTING TRANSGENIC PLANT TISSUES

I. Bar Gene II. Callus-mediated Shoot Formation III. System Based on D-Amino Acid Metabolism IV. Three rol-gene System V. Fluorescent Protein Markers VI. Greenhouse Selection System VII. 2-Deoxyglucose VIII. Mercury Resistance Genes IX. Cyanamide X. Positive Selection Systems XI. Comparison of Three Selection Systems XII. Agrobacterium Increases Resistance to Selective Agents XIII. Systems for Monitoring Plant Gene Expression A. Multiplex PCR Analysis Protocol B. Monitoring Gene Expression at the One Cell Level C. Localizing GUS Expression D. Localizing Single-copy T-DNA Insertion

CHAPTER 6 AGROBACTERIUM RHIZOGENES AND ROL GENES

I. Hairy Root Culture Techniques

A. Nutrient Mist Bioreactors B. Mesh-draft Airlift Reactor C. Alleviating Oxygen Deficiencies D. Effect of Hairyness on Fluid Flow and Oxygen Transfer E. Effects of Low Shear-stress Preculture Conditions F. Simultaneous Monitoring of Morphology & Metabolite Production II. Storage of Hairy Root Cultures III. Regeneration of Plants from Hairy Roots IV. Secondary Metabolites from Hairy Root Cultures V. Effects of rol Genes on In Vitro Morphogenesis A. Bushy Chrysanthemums B. Unique Plant Phenotypes by Differential Expression of rolC C. Stimulation of In Vitro Rooting D. Enhancement of Shoot Regeneration E. Plant Improvement and Micropropagation F. Production of Dwarf Plants V. Truffle Production VI. Phytoremediation VII. Circadian Rhythms in Hairy Root Cultures

CHAPTER 7 OTHER APPLICATIONS OF PLANT TRANSFORMATION

I. Transgenic Composite Plants as Research Tools II. Elimination of Peanut Allergy Using RNAi Technology III. Transgenic Spirodelia as a System for Biotechnology Products IV. In Vitro Culture Enhancements A. Increased Somatic Embryogenesis B. Hemoglobin Gene Enhances In Vitro Growth C. Engineered Polyamine Accumulation Enhances Tomato Quality D. Antifreeze Proteins E. Expression of Frog Skin Peptide Increases Disease Resistance F. Aradopsis Genes Create Novel Ornamental Plant V. Transgenic Plants with Modified Flowering Characteristics VI. High Level Selection of GFP VII. Production of Vaccines VIII. Biofuel from Transgenic Rice Waste Products IX. Pest Resistance X Food Improvements XI. Continuous Nondestructive Production of Recombinant Proteins XII. Induction of Genomic Variations by Genetic Engineering Processes XIII. Monitoring and Bioremediation of Environmental Chemicals XIV.Control of Phytopathogenic Microorganisms XV. Transgenic Spirodela as a System for Biotechnology Products XVI. Safety of Genetically Transformed Plants XVII. Insecticidal Efficacy of Bt Potato

CHAPTER 8 PATENTS

I. Laser-mediated Transformation II. High-velocity Aerosol Beams Introduce Molecules into Plant Cells III. Apparatus and Methods for Transforming Individual Cells IV. Microparticle Bombardment System V. Improved Microprojectile-mediated Transformation VI. Microparticle Bombardment Method and Apparatus VII. Agrobacterium-mediated Transformation of Graminae VIII. Transformation of Adult Woody Plants VIX. Gene Transfer into Pollen by Ion Beam Radiation VX. Process for Microinjecting Genetic Material into Cells VXI. Production of Transformed Doubled Haploid Plants VXII.Turfgrasses That Signal Exposure to Chemicals or Stress

CHAPTER 9 FREE DATABASES

DIRECTORY OF RESEARCH: Includes email and/or fax addresses of all authors

TAXONOMIC INDEX

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